CARD8 Knockout cell line (Jurkat, Clone E6-1)

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location： Home > Products > KO Cell Lines > CARD8 Knockout cell line (Jurkat, Clone E6-1)

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CARD8 Knockout cell line (Jurkat, Clone E6-1) Order

CARD8 Knockout cell line (Jurkat, Clone E6-1)
Catalog#
YKO-H771
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
CARD8 Knockout cell line (Jurkat, Clone E6-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
CARD8 Knockout cell line (Jurkat, Clone E6-1)
Morphology
Lymphocyte-like,suspension
Passage ratio
1:3-1:5
Species
Human
Gene Name
CARD8
Gene ID
22900
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 8 (based on transcript CARD8-231)
Validation: PCR+Sanger Sequencing
CARD8 Knockout cell line (Jurkat, Clone E6-1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Jurkat, Clone E6-1		 STR Info (Cell bank)
Cell Line:Jurkat, Clone E6-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 9999
D13S317 812812
D7S820 812812
D16S539 11111111
VWA 18181818
TH01 69.369.3
AMEL XYXY
TPOX 810810
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CARD8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat, Clone E6-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CARD8 gene knockout CARD8 Knockout cell line (Jurkat, Clone E6-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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